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Registros recuperados : 7 | |
3. | | MENDONÇA, K. S.; MICHEL, G. B.; NALERIO, E. S.; MENDONÇA, M.; CARDOSO, M. R. I.; SILVA, W. P. da. Genotypic profile of Listeria monocytogenes isolated in refrigerated chickens in southern Rio Grande do Sul, Brazil. Ciência Rural, Santa Maria, v. 46, n. 1, p. 132-137, jan. 2016. Biblioteca(s): Embrapa Pecuária Sul. |
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5. | | VAZ, C. S. L.; STRECK, A. F.; MICHAEL, G. B.; MARKS, F. S.; RODRIGUES, D. P.; REIS, E. M. F. dos; CARDOSO, M. R. I.; CANAL, C. W. Antimicrobial resistance and subtyping of Salmonella enterica subspecies enterica serovar Enteritidis isolated from human outbreaks and poultry in southern Brazil. Poultry Science, v. 89, p. 1530-1536, 2010. Projeto/Plano de Ação: 03.08.60.400-03. Biblioteca(s): Embrapa Suínos e Aves. |
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6. | | SANTIAGO-NETO, W.; MACHADO, G.; PAIM, D. S.; CAMPOS, T. de; BRITO, M. A. V. P. e; CARDOSO, M. R. I.; CORBELLINI, L. G. Relação da idade na presença de bactérias resistentes a antimicrobianos em rebanhos leiteiros no Rio Grande do Sul. Pesquisa Veterinária Brasileira, v. 34, n. 7, p. 613-620, 2014. Biblioteca(s): Embrapa Gado de Leite. |
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7. | | SATO, J. P. H.; TAKEUTI, K. L.; ANDRADE, M. R.; KOERICH, P. K. V.; TAGLIARI, V.; BERNARDI, M. L.; CARDOSO, M. R. I.; BARCELLOS, D. E. S. N. Virulence profiles of enterotoxigenic Escherichia coli isolated from piglets with post-weaning diarrhea and classification according to fecal consistency. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 4, p. 253-257, abr. 2016. Biblioteca(s): Embrapa Unidades Centrais. |
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Registros recuperados : 7 | |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
02/05/2016 |
Data da última atualização: |
29/12/2017 |
Autoria: |
SATO, J. P. H.; TAKEUTI, K. L.; ANDRADE, M. R.; KOERICH, P. K. V.; TAGLIARI, V.; BERNARDI, M. L.; CARDOSO, M. R. I.; BARCELLOS, D. E. S. N. |
Afiliação: |
JOSÉ PAULO H. SATO, UFRGS; KARINE L. TAKEUTI, UFRGS; MARIANA R. ANDRADE, UFRGS; PRISCILLA K. V. KOERICH, BRF - Brasil Foods; VINICIUS TAGLIARI, BRF- Brasil Foods; MARI L. BERNARDI, UFRGS; MARISA R. I. CARDOSO, UFRGS; DAVID E. S. N. BARCELLOS, UFRGS. |
Título: |
Virulence profiles of enterotoxigenic Escherichia coli isolated from piglets with post-weaning diarrhea and classification according to fecal consistency. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 4, p. 253-257, abr. 2016. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to assess the frequency and association of virulence factors of Escherichia (E.) coli isolated from weaned piglets with diarrhea and to correlate it with fecal consistency. A total of 152 rectal swabs were collected from 25-40 day-old piglets with diarrhea, in farms of Southern Brazil. Phenotypical and molecular techniques were used for bacterial isolation, characterization and classification of enterotoxigenic E. coli (ETEC) pathotypes. Statistical analysis was carried out to determine the frequency of virulence factors and virotypes, of fimbriae F4, F5, F6, F18, F41 and toxins LT, STa, STb and STx2e. Out of 456 E. coli isolates, 287 (62.9%) samples showed significant growth of E. coli. Among them, 194 (67.6%) samples showed at least one virulence factor, indicating that ETEC is an important etiological agent of diarrhea in weaned piglets. Higher frequencies were found of fimbria F4 and F18 and enterotoxins LT, STa and STb. Significant association was found to F4, LT, STa and STb; between F18 and STa and STx2e; between F5 and LT, STa and STb. The most frequent virotypes were F18-STa, F4-LT-STa-STb, F4-STa, F4-LT-STb and F18-STa-STx2e. Beta-hemolysis was observed in 47.4% of samples and there was significant association between hemolytic samples and virulence factors F4, F18, STa and STx2e. Regarding fecal consistency, there was significant association of liquid feces and F4 fimbria, STa toxin and virotypes F4-STa and F4-F5-LT-STa-STb. Since there was significant association of ETEC and liquid feces in nursery piglets, it is important to prioritize the sampling of liquid feces for the diagnosis etiologic cause of diarrhea. MenosThe aim of this study was to assess the frequency and association of virulence factors of Escherichia (E.) coli isolated from weaned piglets with diarrhea and to correlate it with fecal consistency. A total of 152 rectal swabs were collected from 25-40 day-old piglets with diarrhea, in farms of Southern Brazil. Phenotypical and molecular techniques were used for bacterial isolation, characterization and classification of enterotoxigenic E. coli (ETEC) pathotypes. Statistical analysis was carried out to determine the frequency of virulence factors and virotypes, of fimbriae F4, F5, F6, F18, F41 and toxins LT, STa, STb and STx2e. Out of 456 E. coli isolates, 287 (62.9%) samples showed significant growth of E. coli. Among them, 194 (67.6%) samples showed at least one virulence factor, indicating that ETEC is an important etiological agent of diarrhea in weaned piglets. Higher frequencies were found of fimbria F4 and F18 and enterotoxins LT, STa and STb. Significant association was found to F4, LT, STa and STb; between F18 and STa and STx2e; between F5 and LT, STa and STb. The most frequent virotypes were F18-STa, F4-LT-STa-STb, F4-STa, F4-LT-STb and F18-STa-STx2e. Beta-hemolysis was observed in 47.4% of samples and there was significant association between hemolytic samples and virulence factors F4, F18, STa and STx2e. Regarding fecal consistency, there was significant association of liquid feces and F4 fimbria, STa toxin and virotypes F4-STa and F4-F5-LT-STa-STb. Since there w... Mostrar Tudo |
Palavras-Chave: |
Consistência fecal; Enterotoxigênica; Fatores de virulência; Fecal consistency; Virulence factors. |
Thesagro: |
Diarreia; Escherichia Coli; Suíno. |
Thesaurus NAL: |
Diarrhea; Enterotoxigenic Escherichia coli; Piglets; Swine. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142679/1/Virulence-profiles.pdf
|
Marc: |
LEADER 02748naa a2200349 a 4500 001 2044270 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aSATO, J. P. H. 245 $aVirulence profiles of enterotoxigenic Escherichia coli isolated from piglets with post-weaning diarrhea and classification according to fecal consistency. 260 $c2016 520 $aThe aim of this study was to assess the frequency and association of virulence factors of Escherichia (E.) coli isolated from weaned piglets with diarrhea and to correlate it with fecal consistency. A total of 152 rectal swabs were collected from 25-40 day-old piglets with diarrhea, in farms of Southern Brazil. Phenotypical and molecular techniques were used for bacterial isolation, characterization and classification of enterotoxigenic E. coli (ETEC) pathotypes. Statistical analysis was carried out to determine the frequency of virulence factors and virotypes, of fimbriae F4, F5, F6, F18, F41 and toxins LT, STa, STb and STx2e. Out of 456 E. coli isolates, 287 (62.9%) samples showed significant growth of E. coli. Among them, 194 (67.6%) samples showed at least one virulence factor, indicating that ETEC is an important etiological agent of diarrhea in weaned piglets. Higher frequencies were found of fimbria F4 and F18 and enterotoxins LT, STa and STb. Significant association was found to F4, LT, STa and STb; between F18 and STa and STx2e; between F5 and LT, STa and STb. The most frequent virotypes were F18-STa, F4-LT-STa-STb, F4-STa, F4-LT-STb and F18-STa-STx2e. Beta-hemolysis was observed in 47.4% of samples and there was significant association between hemolytic samples and virulence factors F4, F18, STa and STx2e. Regarding fecal consistency, there was significant association of liquid feces and F4 fimbria, STa toxin and virotypes F4-STa and F4-F5-LT-STa-STb. Since there was significant association of ETEC and liquid feces in nursery piglets, it is important to prioritize the sampling of liquid feces for the diagnosis etiologic cause of diarrhea. 650 $aDiarrhea 650 $aEnterotoxigenic Escherichia coli 650 $aPiglets 650 $aSwine 650 $aDiarreia 650 $aEscherichia Coli 650 $aSuíno 653 $aConsistência fecal 653 $aEnterotoxigênica 653 $aFatores de virulência 653 $aFecal consistency 653 $aVirulence factors 700 1 $aTAKEUTI, K. L. 700 1 $aANDRADE, M. R. 700 1 $aKOERICH, P. K. V. 700 1 $aTAGLIARI, V. 700 1 $aBERNARDI, M. L. 700 1 $aCARDOSO, M. R. I. 700 1 $aBARCELLOS, D. E. S. N. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 4, p. 253-257, abr. 2016.
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